β-Lactamases and β-Lactamase Inhibitors in the 21st Century

DOI: 10.1016/j.jmb.2019.04.002 Authors: Catherine L. Tooke, Philip Hinchliffe, Éilís C. Bragginton, Charlotte K. Colenso, Viivi H. A. Hirvonen, Yuiko Takebayashi, James Spencer Abstract: The β-lactams retain a central place in the antibacterial armamentarium. In...

Determination of the iron(IV) local spin states of the Q intermediate of soluble methane monooxygenase by Kβ X-ray emission spectroscopy

DOI: 10.1007/s00775-022-01953-4 Authors: George E. Cutsail, R. Banerjee, Derek B. Rice, Olivia McCubbin Stepanic, John D. Lipscomb, Serena DeBeer Abstract: Soluble methane monooxygenase (sMMO) facilitates the conversion of methane to methanol at a non-heme FeIV2...

Design of a single protein that spans the entire 2-V range of physiological redox potentials

DOI: 10.1073/pnas.1515897112 Authors: Parisa Hosseinzadeh, Nicholas Marshall, Kelly N. Chacón, Yang Yu, Mark J. Nilges, Siu Yee New, Stoyan A. Tashkov, Ninian J. Blackburn, Yi Lu Abstract: The reduction potential (E°’) is a critical parameter in determining the...

A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis

DOI: 10.1016/j.jmr.2013.01.016 Authors: Royi Kaufmann, Itamar Yadid, Daniella Goldfarb Abstract: N/A

NO Reduction by Nitric-oxide Reductase from Denitrifying Bacterium Pseudomonas aeruginosa

DOI: 10.1074/jbc.m409996200 Authors: Hideyuki Kumita, Koji Matsuura, Tomoya Hino, Satoshi Takahashi, Hiroshi Hori, Yoshihiro Fukumori, Isao Morishima, Yoshitsugu Shiro Abstract: Nitric-oxide reductase (NOR) of a denitrifying bacterium catalyzes NO reduction to N 2 O...

Diiron-cluster intermediates in biological oxygen activation reactions

DOI: 10.1016/s0020-1693(96)05427-8 Authors: Dale E. Edmondson, Boi Hanh Huynh Abstract: N/A

Catalytic transformation of dinitrogen into ammonia and hydrazine by iron-dinitrogen complexes bearing pincer ligand

DOI: 10.1038/ncomms12181 Authors: Shogo Kuriyama, Kazuya Arashiba, Kazunari Nakajima, Yuki Matsuo, Hiromasa Tanaka, Kazuyuki Ishii, Kazunari Yoshizawa, Yoshiaki Nishibayashi Abstract: Synthesis and reactivity of iron-dinitrogen complexes have been extensively studied,...

Millisecond Time-Resolved Solid-State NMR Reveals a Two-Stage Molecular Mechanism for Formation of Complexes between Calmodulin and a Target Peptide from Myosin Light Chain Kinase

DOI: 10.1021/jacs.0c11156 Authors: Jaekyun Jeon, Wai‐Ming Yau, Robert Tycko Abstract: Calmodulin (CaM) mediates a wide range of biological responses to changes in intracellular Ca2+ concentrations through its calcium-dependent binding affinities to numerous target...

Vascular Consequences of Endothelial Nitric Oxide Synthase Uncoupling for the Activity and Expression of the Soluble Guanylyl Cyclase and the cGMP-Dependent Protein Kinase

DOI: 10.1161/01.atv.0000168896.64927.bb Authors: Thomas Münzel, Andreas Daiber, Volker Ullrich, Alexander Mülsch Abstract: Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolemia, hypertension, diabetes mellitus, chronic...

Catalysis-dependent selenium incorporation and migration in the nitrogenase active site iron-molybdenum cofactor

DOI: 10.7554/elife.11620 Authors: Thomas Spatzal, Kathryn Perez, James B. Howard, Douglas C. Rees Abstract: Dinitrogen reduction in the biological nitrogen cycle is catalyzed by nitrogenase, a two-component metalloenzyme. Understanding of the transformation of the...