A Tryptophan Neutral Radical in the Oxidized State of Versatile Peroxidase from Pleurotus eryngii

DOI: 10.1074/jbc.m510424200 Authors: Rebecca Pogni, Maria Camilla Baratto, Christian Teutloff, Stefania Giansanti, Francisco J. Ruiz‐Dueñas, Thomas Choinowski, Klaus Piontek, Ángel T. Martı́nez, Friedhelm Lendzian, Riccardo Basosi Abstract: Versatile peroxidases are...

Reactive Intermediates in Cytochrome P450 Catalysis

DOI: 10.1074/jbc.r113.473108 Authors: Courtney M. Krest, Elizabeth L. Onderko, Timothy H. Yosca, Julio C. Calixto, Richard Karp, Jovan Livada, Jonathan Rittle, Michael T. Green Abstract: Recently, we reported the spectroscopic and kinetic characterizations of...

Cyanide inhibition of cytochrome c oxidase. A rapid-freeze e.p.r. investigation

DOI: 10.1042/bj2240829 Authors: P. Jensen, Michael T. Wilson, Roland Aasa, Bo G. Malmström Abstract: The inhibition of cytochrome c oxidase by cyanide, starting either with the resting or the pulsed enzyme, was studied by rapid-freeze quenching followed by...

Enzymatic Birch Reduction via Hydrogen Atom Transfer at an Aqua-Tungsten-bis-Metallopterin Cofactor

DOI: 10.1021/acscatal.3c01781 Authors: Carola S. Seelmann, Simona G. Huwiler, Martin Culka, Marc J. F. Strampraad, Till Biskup, Stefan Weber, G. Matthias Ullmann, Volker Schünemann, Peter‐Leon Hagedoorn, Antonio J. Pierik, Matthias Boll Abstract: The Birch reduction...

Reversible amorphization and the catalytically active state of crystalline Co3O4 during oxygen evolution

DOI: 10.1038/ncomms9625 Authors: Arno Bergmann, Elías Martínez-Moreno, Detre Teschner, Petko Chernev, Manuel Gliech, Jorge Ferreira de Araújo, Tobias Reier, Holger Dau, Peter Strasser Abstract: Water splitting catalysed by earth-abundant materials is pivotal for...

Probing substrate binding to the metal binding sites in metallo-β-lactamase L1 during catalysis

DOI: 10.1039/c5md00358j Authors: Mahesh Aitha, Sameer Al-Adbul-Wahid, David L. Tierney, Michael W. Crowder Abstract: Metal ions in metallo-β-lactamases (MBLs) play a major role in catalysis. In this study we investigated the role of the metal ions in the Zn1 and Zn2...

Biohybrid photosynthetic charge accumulation detected by flavin semiquinone formation in ferredoxin-NADP⁺reductase

DOI: 10.1039/d2sc01546c Authors: Lisa M. Utschig, Udita Brahmachari, Karen L. Mulfort, Jens Niklas, Oleg G. Poluektov Abstract: Flavin chemistry is ubiquitous in biological systems with flavoproteins engaged in important redox reactions. In photosynthesis, flavin...

Detection of a Kinetically Competent Compound-I Intermediate in the Vancomycin Biosynthetic Enzyme OxyB

DOI: 10.1021/jacs.4c03102 Authors: Andy K. L. Nguy, Ryan J. Martinie, Amanda Cai, Mohammad R. Seyedsayamdost Abstract: Cytochrome P450 enzymes are abundantly encoded in microbial genomes. Their reactions have two general outcomes, one involving oxygen insertion via a...

Binuclear Manganese Compounds of Potential Biological Significance. Part 2. Mechanistic Study of Hydrogen Peroxide Disproportionation by Dimanganese Complexes:  The Two Oxygen Atoms of the Peroxide End up in a Dioxo Intermediate

DOI: 10.1021/ic020646n Authors: Lionel Dubois, Régis Caspar, Lilian Jacquamet, P. Petit, Marie‐France Charlot, Carole Baffert, Marie‐Noëlle Collomb, Alain Deronzier, Jean‐Marc Latour Abstract: The dimanganese(II,II) complexes 1a [Mn(2)(L)(OAc)(2)(CH(3)OH)](ClO(4)) and...

CW and Pulse EPR of Cytochrome P450 to Determine Structure and Function

DOI: 10.1007/978-3-319-59100-1_5 Authors: Joshua S. Harbort, James J. De Voss, Jeanette E. Stok, Stephen G. Bell, Jeffrey R. Harmer Abstract: N/A