A New Reaction for Improved Calibration of EPR Rapid-Freeze Quench Times: Kinetics of Ethylene Diamine Tetraacetate (EDTA) Transfer from Calcium(II) to Copper(II)

DOI: 10.1007/s00723-021-01448-6 Authors: Abigail L. Schroeter, Hao Yang, Christopher D. James, Brian M. Hoffman, Peter E. Doan Abstract: N/A

Gemcitabine 5‘-Triphosphate Is a Stoichiometric Mechanism-Based Inhibitor of Lactobacillus leichmannii Ribonucleoside Triphosphate Reductase:  Evidence for Thiyl Radical-Mediated Nucleotide Radical Formation

DOI: 10.1021/bi972934e Authors: Domingos J. Silva, JoAnne Stubbe, Vicente Sámano, Morris J. Robins Abstract: Ribonucleoside triphosphate reductase (RTPR) from Lactobacillus leichmannii utilizes adenosylcobalamin and catalyzes the conversion of nucleoside triphosphates...

Pre-Steady-State Reactivity of Peptidylglycine Monooxygenase Implicates Ascorbate in Substrate Triggering of the Active Conformer

DOI: 10.1021/acs.biochem.2c00080 Authors: Evan F. Welch, Katherine W. Rush, Renee J. Arias, Ninian J. Blackburn Abstract: Peptidylglycine monooxygenase (PHM) is essential for the posttranslational amidation of neuroendocrine peptides. An important aspect of the PHM...

Kinetic and Spectroscopic Characterization of the Catalytic Ternary Complex of Tryptophan 2,3-Dioxygenase

DOI: 10.1021/acs.biochem.0c00179 Authors: Jiafeng Geng, Andrew C. Weitz, Kednerlin Dornevil, Michael P. Hendrich, Aimin Liu Abstract: The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan...

Use of 2,3,5-F₃Y-β2 and 3-NH₂Y-α2 To Study Proton-Coupled Electron Transfer in Escherichia coli Ribonucleotide Reductase

DOI: 10.1021/bi101319v Authors: Mohammad R. Seyedsayamdost, Cyril S. Yee, JoAnne Stubbe Abstract: Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleoside 5′-diphosphates (NDPs) to deoxynucleotides (dNDPs). The...

Methane Monooxygenase

DOI: 10.1007/978-94-011-0255-1_1 Authors: Stephen J. Lippard Abstract: N/A

Violazione di norme imperative e nullità del contratto

DOI: 10.1016/j.bbrc.2005.08.166 Authors: Antonio Albanese Abstract: Since the discovery of cytochromes P450 and their assignment to heme proteins a reactive iron-oxo intermediate as the hydroxylating species has been discussed. It is believed that the electronic...

Revisiting the Val/Ile Mutation in Mammalian and Bacterial Nitric Oxide Synthases: A Spectroscopic and Kinetic Study

DOI: 10.1021/acs.biochem.6b01018 Authors: Marine Weisslocker-Schaetzel, Mehdi Lembrouk, Jérôme Santolini, Pierre Dorlet Abstract: Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a...

Shewanella oneidensis Cytochrome c Nitrite Reductase (ccNiR) Does Not Disproportionate Hydroxylamine to Ammonia and Nitrite, Despite a Strongly Favorable Driving Force

DOI: 10.1021/bi401705d Authors: Matthew D. Youngblut, Daniel J. Pauly, Natalia Stein, Daniel T. Walters, John A. Conrad, Graham R. Moran, Brian Bennett, A. Andrew Pacheco Abstract: Cytochrome c nitrite reductase (ccNiR) from Shewanella oneidensis, which catalyzes the...

Evidence for a Long-Lived, Cu-Coupled and Oxygen-Inert Disulfide Radical Anion in the Assembly of Metallothionein-3 Cu(I)₄-Thiolate Cluster

DOI: 10.1021/jacs.1c03984 Authors: Jenifer S. Calvo, Rhiza Lyne E. Villones, Nicholas J. York, Ewelina Stefaniak, Grace E. Hamilton, Allison L. Stelling, Wojciech Bal, Brad S. Pierce, Gabriele Meloni Abstract: The human copper-binding protein metallothionein-3 (MT-3)...