Horseradish Peroxidase Phe172→ Tyr Mutant

DOI: 10.1074/jbc.270.31.18413 Authors: Vaughn P. Miller, David B. Goodin, Alan E. Friedman, Christa Hartmann, Paul R. Ortiz de Montellano Abstract: A gene coding for the F172Y mutant of horseradish peroxidase isozyme C (HRP) has been constructed and expressed in both...

Decarboxylation involving a ferryl, propionate, and a tyrosyl group in a radical relay yields heme b

DOI: 10.1074/jbc.ra117.000830 Authors: Bennett R. Streit, Arianna I. Celis, Garrett C. Moraski, Krista A. Shisler, Eric M. Shepard, Kenton R. Rodgers, Gudrun S. Lukat-Rodgers, Jennifer L. DuBois Abstract: The H2O2-dependent oxidative decarboxylation of coproheme III...

Use of Noncanonical Tyrosine Analogues to Probe Control of Radical Intermediates during Endoperoxide Installation by Verruculogen Synthase (FtmOx1)

DOI: 10.1021/acscatal.2c01037 Authors: Chi‐Yun Lin, Angel L. Muñoz Hernández, Tatiana N. Laremore, Alexey Silakov, Carsten Krebs, Amie K. Boal, J. Martin Bollinger Abstract: Important bioactive natural products, including prostaglandin H2 and artemisinin, contain...

The Mechanisms of Catalysis by Metallo β‐Lactamases

DOI: 10.1155/2008/576297 Authors: Michael I. Page, Adriana Badarau Abstract: Class B beta-lactamases or metallo-beta-lactamases (MBLs) require zinc ions to catalyse the hydrolysis of beta-lactam antibiotics such as penicillins, cephalosporins, carbapenems, and...

On-grid and in-flow mixing for time-resolved cryo-EM

DOI: 10.1107/s2059798321008810 Authors: David P. Klebl, Howard D. White, Frank Sobott, Stephen P. Muench Abstract: Time-resolved cryo-electron microscopy (TrEM) allows the study of proteins under non-equilibrium conditions on the millisecond timescale, permitting the...

EPR of Short-Lived Magnetic Species

DOI: 10.1007/978-3-030-39668-8_10 Authors: Vincent Maurel, S. Gambarelli Abstract: N/A

Mechanistic studies on the dehydrogenases of methylotrophic bacteria. 1. The influence of substrate binding to reduced trimethylamine dehydrogenase on the intramolecular electron transfer between its prosthetic groups

DOI: 10.1042/bj2070233 Authors: Daniël J. Steenkamp, Helmut Beinert Abstract: The trimethylamine dehydrogenase of bacterium W3A1 is reduced with the formation of a triplet state in which two electrons, derived from the substrate, are distributed between the [4Fe-4S]...

Structural and Kinetic Studies on Metallo-β-lactamase IMP-1

DOI: 10.1021/bi200839h Authors: Dionne H. Griffin, Timothy Richmond, Carlo Sanchez, Abraham Moller, Robert M. Breece, David L. Tierney, Brian Bennett, Michael W. Crowder Abstract: In an effort to probe for metal binding to metallo-β-lactamase (MβL) IMP-1, the enzyme...

Probing the early activation mechanism of olefin oligomerisation catalysis using molybdenum, scandium and chromium halide complexes

DOI: OA:W191742290 Authors: Stuart A. Bartlett Abstract: The efficient catalytic conversion of small molecules into more complex species by clean, energy efficient processes is of high commercial demand. The selective trimerisation and tetramerisation of ethene to...

The Modified Q-cycle Explains the Apparent Mismatch between the Kinetics of Reduction of Cytochromes c 1 and b H in the bc 1 Complex

DOI: 10.1074/jbc.m305461200 Authors: Antony R. Crofts, Vladimir P. Shinkarev, Derrick R.J. Kolling, Sangjin Hong Abstract: Crystallographic structures of the bc1 complex from different sources have provided evidence that a movement of the Rieske iron-sulfur protein...