Extended depth-of-field microscopy with a high-speed deformable mirror

DOI:

10.1364/ol.42.000995

Authors:

William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas G. Bifano, Jérôme Mertz

Abstract:

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.