Freeze-quenched iron-oxo intermediates in cytochromes P450

DOI: 10.1016/j.bbrc.2005.08.166 Authors: Christiane Jung, Volker Schünemann, Friedhelm Lendzian Abstract: N/A

Simultaneous reduction of iron–sulfur protein and cytochrome b _L during ubiquinol oxidation in cytochrome bc ₁ complex

DOI: 10.1073/pnas.0607812104 Authors: Jian Zhu, Tsuyoshi Egawa, Syun‐Ru Yeh, Linda Yu, Chang‐An Yu Abstract: The key step of the protonmotive Q-cycle mechanism of the cytochrome bc(1) complex is the bifurcated oxidation of ubiquinol at the Qp site. It was postulated...

(μ-1,2-Peroxo)diiron(III/III) Complex as a Precursor to the Diiron(III/IV) Intermediate X in the Assembly of the Iron-Radical Cofactor of Ribonucleotide Reductase from Mouse

DOI: 10.1021/bi061717n Authors: Danny Yun, Ricardo García‐Serres, Brandon M. Chicalese, Young Hun An, Boi Hanh Huynh, J. Martin Bollinger Abstract: Stopped-flow absorption and freeze-quench electron paramagnetic resonance (EPR) and Mössbauer spectroscopies have been...

Low-cost stopped-flow and freeze-quench device for double mixing

DOI: 10.1016/j.ohx.2023.e00409 Authors: Łukasz Bujnowicz, Rafał Pietras, Marcin Sarewicz, Artur Osyczka Abstract: Experiments based on fast reagent mixing and observation of reaction progress are considered a powerful tool for investigating the kinetics of chemical...

Critical Comparison of the Superoxide Dismutase-like Activity of Carbon Antioxidant Nanozymes by Direct Superoxide Consumption Kinetic Measurements

DOI: 10.1021/acsnano.9b04229 Authors: Gang Wu, Vladimír Berka, Paul J. Derry, Kimberly Mendoza, Eugenia Kakadiaris, Trenton Roy, Thomas A. Kent, James M. Tour, Ah‐Lim Tsai Abstract: The superoxide dismutase-like activity of poly(ethylene glycolated) hydrophilic carbon...

Spectroscopic and Mechanistic Studies of Heterodimetallic Forms of Metallo-β-lactamase NDM-1

DOI: 10.1021/ja410376s Authors: Hao Yang, Mahesh Aitha, Amy R. Marts, Alyssa Hetrick, Brian Bennett, Michael W. Crowder, David L. Tierney Abstract: In an effort to characterize the roles of each metal ion in metallo-β-lactamase NDM-1, heterodimetallic analogues...

Transient Free Radicals in Iron/Oxygen Reconstitution of Mutant Protein R2 Y122F.

DOI: 10.1074/jbc.270.21.12361 Authors: Margareta Sahlin, G. Laßmann, Stephan Pötsch, Britt‐Marie Sjöberg, Astrid Gräslund Abstract: Ferrous iron/oxygen reconstitution of the mutant R2 apoprotein Y122F leads to formation of a diferric center similar to that of the...

[7] Freeze-quench and chemical-quench techniques

DOI: 10.1016/s0076-6879(78)54010-x Authors: David P. Ballou Abstract: N/A

Role of the Zn₁and Zn₂sites in Metallo-β-lactamase L1

DOI: 10.1021/ja8035916 Authors: Zhenxin Hu, Gopalraj Periyannan, Brian Bennett, Michael W. Crowder Abstract: In an effort to probe the role of the Zn(II) sites in metallo-beta-lactamase L1, mononuclear metal ion containing and heterobimetallic analogues of the enzyme...

Dioxygen Activation at Non-Heme Diiron Centers:  Characterization of Intermediates in a Mutant Form of Toluene/o-Xylene Monooxygenase Hydroxylase

DOI: 10.1021/ja062762l Authors: Leslie J. Murray, Ricardo García‐Serres, Sunil Naik, Boi Hanh Huynh, Stephen J. Lippard Abstract: We report the generation and characterization of an intermediate in a mutant form of the toluene/o-xylene monooxygenase hydroxylase...