Characterization of the Intramolecular Electron Transfer Pathway from 2-Hydroxyphenazine to the Heterodisulfide Reductase fromMethanosarcina thermophila

DOI: 10.1074/jbc.m004809200 Authors: Eisuke Murakami, Uwe Deppenmeier, Stephen W. Ragsdale Abstract: Heterodisulfide reductase (HDR) is a component of the energy-conserving electron transfer system in methanogens. HDR catalyzes the two-electron reduction of coenzyme...

The Production of Nitrous Oxide by the Heme/Nonheme Diiron Center of Engineered Myoglobins (Fe_BMbs) Proceeds through a trans-Iron-Nitrosyl Dimer

DOI: 10.1021/ja410542z Authors: Hirotoshi Matsumura, Takahiro Hayashi, Saumen Chakraborty, Yi Lu, Pierre Moënne‐Loccoz Abstract: Denitrifying NO reductases are transmembrane protein complexes that are evolutionarily related to heme/copper terminal oxidases. They...

Oxidation of (horse) hemoglobin by copper: an intermediate detected by electron spin resonance

DOI: 10.1021/bi00585a005 Authors: Joseph M. Rifkind Abstract: The oxidation of horse hemoglobin by Cu(II) has been followed by the changes in the electron spin resonance spectra of copper. By stopped-flow and freeze-quenching techniques, it is shown that the...

Kinetic Characterization of an Organic Radical in the Ascarylose Biosynthetic Pathway

DOI: 10.1021/bi961370w Authors: David A. Johnson, George Gassner, Vahe Bandarian, Frank J. Ruzicka, David P. Ballou, George H. Reed, Hung‐wen Liu Abstract: The lipopolysaccharide of Yersinia pseudotuberculosis V includes a 3,6-dideoxyhexose, ascarylose, as the...

Oxygen Activation by a Mixed-Valent, Diiron(II/III) Cluster in the Glycol Cleavage Reaction Catalyzed by myo-Inositol Oxygenase

DOI: 10.1021/bi0526276 Authors: Gang Xing, Eric W. Barr, Yinghui Diao, Lee M. Hoffart, K. Sandeep Prabhu, Ryan J. Arner, C. Channa Reddy, Carsten Krebs, J. Martin Bollinger Abstract: myo-Inositol oxygenase (MIOX) catalyzes the ring-cleaving, four-electron oxidation of...

Redox Function of Tetrahydrobiopterin and Effect of l-Arginine on Oxygen Binding in Endothelial Nitric Oxide Synthase

DOI: 10.1021/bi049026j Authors: Vladimír Berka, Hui-Chun Yeh, De Gao, Kiran Farheen, Ah‐Lim Tsai Abstract: Tetrahydrobiopterin (BH(4)), not dihydrobiopterin or biopterin, is a critical element required for NO formation by nitric oxide synthase (NOS). To elucidate how...

Dioxygen Activation at Non-Heme Diiron Centers:  Oxidation of a Proximal Residue in the I100W Variant of Toluene/o-Xylene Monooxygenase Hydroxylase

DOI: 10.1021/bi7017128 Authors: Leslie J. Murray, Ricardo García‐Serres, Michael S. McCormick, Roman Davydov, Sunil Naik, Sun‐Hee Kim, Brian M. Hoffman, Boi Hanh Huynh, Stephen J. Lippard Abstract: At its carboxylate-bridged diiron active site, the hydroxylase...

O–H Activation by an Unexpected Ferryl Intermediate during Catalysis by 2-Hydroxyethylphosphonate Dioxygenase

DOI: 10.1021/jacs.6b12147 Authors: Spencer C. Peck, Chen Wang, Laura M. K. Dassama, Bo Zhang, Yisong Guo, Lauren J. Rajakovich, J. Martin Bollinger, Carsten Krebs, Wilfred A. van der Donk Abstract: Activation of O-H bonds by inorganic metal-oxo complexes has been...

Rapid kinetics investigations of peracid oxidation of ferric cytochrome P450cam: Nature and possible function of compound ES☆

DOI: 10.1016/j.jinorgbio.2006.09.026 Authors: Tatyana Spolitak, John H. Dawson, David P. Ballou Abstract: N/A

Spectroscopic and Mechanistic Investigations of Dehaloperoxidase B fromAmphitrite ornata

DOI: 10.1021/bi100407v Authors: Jennifer D’Antonio, E.L. D'Antonio, Matthew K. Thompson, Edmond F. Bowden, Stefan Franzen, Tatyana I. Smirnova, Reza A. Ghiladi Abstract: Dehaloperoxidase (DHP) from the terebellid polychaete Amphitrite ornata is a bifunctional...