An Oxyferrous Heme/Protein-based Radical Intermediate Is Catalytically Competent in the Catalase Reaction of Mycobacterium tuberculosis Catalase-Peroxidase (KatG)

DOI: 10.1074/jbc.m808106200 Authors: Javier Suárez, Kalina Ranguelova, Andrzej A. Jarzęcki, J. Manzerova, Vladimir Krymov, Xiangbo Zhao, Shengwei Yu, Leonid Metlitsky, Gary J. Gerfen, Richard S. Magliozzo Abstract: A mechanism accounting for the robust catalase...

Direct Spectroscopic and Kinetic Evidence for the Involvement of a Peroxodiferric Intermediate during the Ferroxidase Reaction in Fast Ferritin Mineralization

DOI: 10.1021/bi980847w Authors: Alice S. Pereira, William Small, Carsten Krebs, Pedro Tavares, Dale E. Edmondson, Elizabeth C. Theil, Boi Hanh Huynh Abstract: Rapid freeze-quench (RFQ) Mössbauer and stopped-flow absorption spectroscopy were used to monitor the...

The Ferroxidase Reaction of Ferritin Reveals a Diferric μ-1,2 Bridging Peroxide Intermediate in Common with Other O₂-Activating Non-Heme Diiron Proteins

DOI: 10.1021/bi990095l Authors: Pierre Moënne‐Loccoz, Carsten Krebs, Kara Herlihy, Dale E. Edmondson, Elizabeth C. Theil, Boi Hanh Huynh, Thomas M. Loehr Abstract: Ferritins are ubiquitous proteins that concentrate, store, and detoxify intracellular iron through...

Probing the Reaction Mechanism of the d-ala-d-ala Dipeptidase, VanX, by Using Stopped-Flow Kinetic and Rapid-Freeze Quench EPR Studies on the Co(II)-Substituted Enzyme

DOI: 10.1021/ja0627343 Authors: Megan L. Matthews, Gopalraj Periyannan, Christine E. Hajdin, Tara K. Sidgel, Brian Bennett, Michael W. Crowder Abstract: In an effort to probe the reaction mechanism of VanX, the d-ala-d-ala dipeptidase required for high-level...

Mechanism of Assembly of the Tyrosyl Radical-Dinuclear Iron Cluster Cofactor of Ribonucleotide Reductase

DOI: 10.1126/science.1650033 Authors: J. Martin Bollinger, Dale E. Edmondson, B.H. Huynh, Jonathan Filley, Jack R. Norton, J. Stubbe Abstract: Incubation of the apoB2 subunit of Escherichia coli ribonucleotide reductase with Fe2+ and O2 produces native B2, which...