Transient Free Radicals in Iron/Oxygen Reconstitution of Mutant Protein R2 Y122F.

DOI: 10.1074/jbc.270.21.12361 Authors: Margareta Sahlin, G. Laßmann, Stephan Pötsch, Britt‐Marie Sjöberg, Astrid Gräslund Abstract: Ferrous iron/oxygen reconstitution of the mutant R2 apoprotein Y122F leads to formation of a diferric center similar to that of the...

[7] Freeze-quench and chemical-quench techniques

DOI: 10.1016/s0076-6879(78)54010-x Authors: David P. Ballou Abstract: N/A

Role of the Zn₁and Zn₂sites in Metallo-β-lactamase L1

DOI: 10.1021/ja8035916 Authors: Zhenxin Hu, Gopalraj Periyannan, Brian Bennett, Michael W. Crowder Abstract: In an effort to probe the role of the Zn(II) sites in metallo-beta-lactamase L1, mononuclear metal ion containing and heterobimetallic analogues of the enzyme...

Dioxygen Activation at Non-Heme Diiron Centers:  Characterization of Intermediates in a Mutant Form of Toluene/o-Xylene Monooxygenase Hydroxylase

DOI: 10.1021/ja062762l Authors: Leslie J. Murray, Ricardo García‐Serres, Sunil Naik, Boi Hanh Huynh, Stephen J. Lippard Abstract: We report the generation and characterization of an intermediate in a mutant form of the toluene/o-xylene monooxygenase hydroxylase...

Reductive half-reaction of xanthine oxidase with xanthine. Observation of a spectral intermediate attributable to the molybdenum center in the reaction of enzyme with xanthine.

DOI: 10.1016/s0021-9258(18)54112-0 Authors: J.H. Kim, Russ Hille Abstract: The reductive half-reaction of xanthine oxidase with substoichiometric concentrations of xanthine and 1-methylxanthine at pH 10 and 8.5 has been examined by UV-visible stopped-flow and...