Rapid Formation of Compound II and a Tyrosyl Radical in the Y229F Mutant of Mycobacterium tuberculosis Catalase-peroxidase Disrupts Catalase but Not Peroxidase Function

DOI: 10.1074/jbc.m304757200 Authors: Shengwei Yu, Stefania Girotto, Xiangbo Zhao, Richard S. Magliozzo Abstract: Catalase-peroxidases (KatG), which belong to Class I heme peroxidase enzymes, have high catalase activity and substantial peroxidase activity. The Y229F...

Trapping and Characterization of a Reaction Intermediate in Carbapenem Hydrolysis by B. cereus Metallo-β-lactamase

DOI: 10.1021/ja801169j Authors: Mariana F. Tioni, Leticia I. Llarrull, Andrés A. Poeylaut-Palena, Marcelo A. Martí, Miguel Saggu, Gopal R. Periyannan, Ernesto G. Mata, Brian Bennett, Daniel H. Murgida, Alejandro J. Vila Abstract: Metallo-beta-lactamases hydrolyze most...

RETRACTED ARTICLE: Endoperoxide formation by an α-ketoglutarate-dependent mononuclear non-haem iron enzyme

DOI: 10.1038/nature15519 Authors: Wupeng Yan, Heng Song, Fuhang Song, Yisong Guo, Cheng-Hsuan Wu, Ampon Sae Her, Yi Pu, Shu Wang, Nathchar Naowarojna, Andrew C. Weitz, Michael P. Hendrich, Catherine E. Costello, Lixin Zhang, Pinghua Liu, Yan Zhang Abstract:...

Reaction of Ferric Cytochrome P450cam with Peracids

DOI: 10.1074/jbc.m501761200 Authors: Tatyana Spolitak, John H. Dawson, David P. Ballou Abstract: Reactions of substrate-free ferric cytochrome P450cam with peracids to generate Fe=O intermediates have previously been investigated with contradictory results. Using...

Mechanistic Studies on the Mononuclear Zn^II-Containing Metallo-β-lactamase ImiS from Aeromonas sobria

DOI: 10.1021/bi060893t Authors: Narayan Prasad Sharma, Christine E. Hajdin, Sowmya Chandrasekar, Brian Bennett, Ke‐Wu Yang, Michael W. Crowder Abstract: In an effort to understand the reaction mechanism of a B2 metallo-beta-lactamase, steady-state and pre-steady-state...