Cross-linking of dicyclotyrosine by the cytochrome P450 enzyme CYP121 from Mycobacterium tuberculosis proceeds through a catalytic shunt pathway

DOI: 10.1074/jbc.m117.794099 Authors: Kednerlin Dornevil, Ian Davis, Andrew J. Fielding, James R. Terrell, Li Ma, Aimin Liu Abstract: CYP121, the cytochrome P450 enzyme in Mycobacterium tuberculosis that catalyzes a single intramolecular C-C cross-linking reaction in...

Fast Kinetics—Stopped-Flow and Rapid Freeze-Quench

DOI: 10.1007/978-3-030-28247-9_13 Authors: Pedro Tavares, Alice S. Pereira Abstract: N/A

Reactivity of Deoxy- and Oxyferrous Dehaloperoxidase B from Amphitrite ornata: Identification of Compound II and Its Ferrous–Hydroperoxide Precursor

DOI: 10.1021/bi200311u Authors: Jennifer D’Antonio, Reza A. Ghiladi Abstract: Dehaloperoxidase (DHP) from the terebellid polychaete Amphitrite ornata is a bifunctional enzyme that possesses both hemoglobin and peroxidase activities. The bifunctional nature of DHP as a...

Characterization of Y122F R2 of Escherichia coli Ribonucleotide Reductase by Time-Resolved Physical Biochemical Methods and X-ray Crystallography^,

DOI: 10.1021/bi9728811 Authors: Wing H. Tong, Doug Burdi, P. Riggs-Gelasco, S. Chen, Dale E. Edmondson, B.H. Huynh, J. Stubbe, Seungil Han, A.S. Arvai, John A. Tainer Abstract: Ribonucleotide reductase (RNR) from Escherichia coli catalyzes the conversion of...

Pre-steady-state Kinetic Analysis of Recombinant Arabidopsis NADH:Nitrate Reductase

DOI: 10.1074/jbc.m100356200 Authors: Lawrie Skipper, Wilbur Campbell, Jeffrey A. Mertens, David J. Lowe Abstract: Recombinant Arabidopsis NADH:nitrate reductase was expressed in Pichia pastoris using fermentation. Large enzyme quantities were purified for...