The Production of Nitrous Oxide by the Heme/Nonheme Diiron Center of Engineered Myoglobins (Fe_BMbs) Proceeds through a trans-Iron-Nitrosyl Dimer

DOI: 10.1021/ja410542z Authors: Hirotoshi Matsumura, Takahiro Hayashi, Saumen Chakraborty, Yi Lu, Pierre Moënne‐Loccoz Abstract: Denitrifying NO reductases are transmembrane protein complexes that are evolutionarily related to heme/copper terminal oxidases. They...

Oxidation of (horse) hemoglobin by copper: an intermediate detected by electron spin resonance

DOI: 10.1021/bi00585a005 Authors: Joseph M. Rifkind Abstract: The oxidation of horse hemoglobin by Cu(II) has been followed by the changes in the electron spin resonance spectra of copper. By stopped-flow and freeze-quenching techniques, it is shown that the...

Kinetic Characterization of an Organic Radical in the Ascarylose Biosynthetic Pathway

DOI: 10.1021/bi961370w Authors: David A. Johnson, George Gassner, Vahe Bandarian, Frank J. Ruzicka, David P. Ballou, George H. Reed, Hung‐wen Liu Abstract: The lipopolysaccharide of Yersinia pseudotuberculosis V includes a 3,6-dideoxyhexose, ascarylose, as the...

Oxygen Activation by a Mixed-Valent, Diiron(II/III) Cluster in the Glycol Cleavage Reaction Catalyzed by myo-Inositol Oxygenase

DOI: 10.1021/bi0526276 Authors: Gang Xing, Eric W. Barr, Yinghui Diao, Lee M. Hoffart, K. Sandeep Prabhu, Ryan J. Arner, C. Channa Reddy, Carsten Krebs, J. Martin Bollinger Abstract: myo-Inositol oxygenase (MIOX) catalyzes the ring-cleaving, four-electron oxidation of...

Redox Function of Tetrahydrobiopterin and Effect of l-Arginine on Oxygen Binding in Endothelial Nitric Oxide Synthase

DOI: 10.1021/bi049026j Authors: Vladimír Berka, Hui-Chun Yeh, De Gao, Kiran Farheen, Ah‐Lim Tsai Abstract: Tetrahydrobiopterin (BH(4)), not dihydrobiopterin or biopterin, is a critical element required for NO formation by nitric oxide synthase (NOS). To elucidate how...