Oxygen-Induced Radical Intermediates in the nNOS Oxygenase Domain Regulated by l-Arginine, Tetrahydrobiopterin, and Thiol

DOI: 10.1021/bi701677r Authors: Vladimír Berka, Lee-Ho Wang, Ah-Lim Tsai Abstract: Fully coupled nitric oxide synthase (NOS) catalyzes formation of nitric oxide (NO), l-citrulline, NADP+, and water from l-arginine, NADPH, and oxygen. Uncoupled or partially coupled NOS...

Substrate Binding and Reduction of Benzoyl-CoA Reductase:  Evidence for Nucleotide-Dependent Conformational Changes

DOI: 10.1021/bi0358871 Authors: Henrik Möbitz, Thorsten Friedrich, Matthias Boll Abstract: Benzoyl-CoA reductase (BCR) from the denitrifying bacterium Thauera aromatica catalyzes the ATP driven two-electron reduction of the aromatic moiety of benzoyl-CoA (BCoA) to a...

Substrate-Mediated Oxygen Activation by Homoprotocatechuate 2,3-Dioxygenase: Intermediates Formed by a Tyrosine 257 Variant

DOI: 10.1021/bi301114x Authors: Michael M. Mbughuni, Katlyn K. Meier, Eckard Münck, John D. Lipscomb Abstract: Homoprotocatechuate (HPCA; 3,4-dihydroxyphenylacetate or 4-carboxymethyl catechol) and O(2) bind in adjacent ligand sites of the active site Fe(II) of...

Nitric Oxide Dioxygenation Reaction in DevS and the Initial Response to Nitric Oxide in Mycobacterium tuberculosis

DOI: 10.1021/bi1015315 Authors: Erik T. Yukl, Alexandra Ioanoviciu, Santhosh Sivaramakrishnan, Michiko Nakano, Paul R. Ortiz de Montellano, Pierre Moënne‐Loccoz Abstract: DevS and DosT from Mycobacterium tuberculosis (MTB) are paralogous heme-based sensor kinases that...

Primary Steps of the Na+-translocating NADH:Ubiquinone Oxidoreductase Catalytic Cycle Resolved by the Ultrafast Freeze-Quench Approach

DOI: 10.1074/jbc.m808984200 Authors: Alexander V. Bogachev, Nikolai Belevich, Yulia V. Bertsova, Michael I. Verkhovsky Abstract: The Na(+)-translocating NADH:ubiquinone oxidoreductase (Na(+)-NQR) is a component of respiratory chain of various bacteria, and it...