Facile Electron Transfer during Formation of Cluster X and Kinetic Competence of X for Tyrosyl Radical Production in Protein R2 of Ribonucleotide Reductase from Mouse

DOI: 10.1021/bi011797p Authors: Danny Yun, Carsten Krebs, Govind P. Gupta, David F. Iwig, Boi Hanh Huynh, J. Martin Bollinger Abstract: The kinetics and mechanism of formation of the tyrosyl radical and mu-(oxo)diiron(III) cluster in the R2 subunit of ribonucleotide...

1.22 Rapid Mixing Techniques for the Study of Enzyme Catalysis

DOI: 10.1016/b978-0-12-374920-8.00127-2 Authors: Sandra Mitić, S. de Vries Abstract: N/A

Substrate Binding Is the Rate-limiting Step in Thromboxane Synthase Catalysis

DOI: 10.1074/jbc.m009177200 Authors: Lee-Ho Wang, Ah‐Lim Tsai, Pei-Yung Hsu Abstract: Thromboxane synthase (TXAS) is a « non-classical » cytochrome P450. Without any need for an external electron donor, or for a reductase or molecular oxygen, it uses prostaglandin H2...

Freeze‐Quench Kinetics

DOI: 10.1002/0470862106.ia340 Authors: Simon de Vries Abstract: Abstract Rapid‐mixing, rapid‐freezing techniques are employed for the study of enzyme or chemical catalytic mechanisms. The techniques aim to provide electronic and molecular structures of transient...

A New Reaction for Improved Calibration of EPR Rapid-Freeze Quench Times: Kinetics of Ethylene Diamine Tetraacetate (EDTA) Transfer from Calcium(II) to Copper(II)

DOI: 10.1007/s00723-021-01448-6 Authors: Abigail L. Schroeter, Hao Yang, Christopher D. James, Brian M. Hoffman, Peter E. Doan Abstract: N/A