NMR at Low and Ultralow Temperatures

DOI: 10.1021/ar300358z Authors: Robert Tycko Abstract: Solid state nuclear magnetic resonance (NMR) measurements at low temperatures have been common in physical sciences for many years and are becoming increasingly important in studies of biomolecular systems. This...

Design, Implementation, Simulation, and Visualization of a Highly Efficient RIM Microfluidic Mixer for Rapid Freeze-Quench of Biological Samples

DOI: 10.1007/s00723-011-0195-7 Authors: Bryan Schmidt, Goher Mahmud, Siowling Soh, Sun Hee Kim, Taylor A. Page, Thomas V. O’Halloran, Bartosz A. Grzybowski, Brian M. Hoffman Abstract: N/A

Tyrosyl Radicals in Dehaloperoxidase

DOI: 10.1074/jbc.m113.496497 Authors: Rania Dumarieh, Jennifer D’Antonio, Alexandria Deliz Liang, Tatyana I. Smirnova, Dimitri A. Svistunenko, Reza A. Ghiladi Abstract: Dehaloperoxidase (DHP) from Amphitrite ornata, having been shown to catalyze the hydrogen...

A Synthetic Single-Site Fe Nitrogenase: High Turnover, Freeze-Quench ⁵⁷Fe Mössbauer Data, and a Hydride Resting State

DOI: 10.1021/jacs.6b01706 Authors: Trevor J. Del Castillo, Niklas B. Thompson, Jonas C. Peters Abstract: The mechanisms of the few known molecular nitrogen-fixing systems, including nitrogenase enzymes, are of much interest but are not fully understood. We recently...

Climbing Nitrogenase: Toward a Mechanism of Enzymatic Nitrogen Fixation

DOI: 10.1021/ar8002128 Authors: Brian M. Hoffman, Dennis R. Dean, Lance C. Seefeldt Abstract: « Nitrogen fixation », the reduction of dinitrogen (N2) to two ammonia (NH3) molecules, by the Mo-dependent nitrogenase is essential for all life. Despite four decades of...