Rapid Kinetic Studies of Acetyl-CoA Synthesis:  Evidence Supporting the Catalytic Intermediacy of a Paramagnetic NiFeC Species in the Autotrophic Wood−Ljungdahl Pathway

DOI: 10.1021/bi011687i Authors: Javier Seravalli, Manoj Kumar, Stephen W. Ragsdale Abstract: CO dehydrogenase/acetyl-CoA synthase (CODH/ACS), a key enzyme in the Wood-Ljungdahl pathway of anaerobic CO(2) fixation, is a bifunctional enzyme containing CODH, which...

Reaction of variant sperm-whale myoglobins with hydrogen peroxide: the effects of mutating a histidine residue in the haem distal pocket

DOI: 10.1042/bj3260109 Authors: Thomas Brittain, Adam Baker, Clive S. Butler, Richard Little, David Lowe, C Greenwood, Nicholas J. Watmough Abstract: The reaction of hydrogen peroxide with a number of variants of sperm-whale myoglobin in which the distal pocket...

Mechanism of the Clostridium thermoaceticum Pyruvate:Ferredoxin Oxidoreductase:  Evidence for the Common Catalytic Intermediacy of the Hydroxyethylthiamine Pyropyrosphate Radical

DOI: 10.1021/bi970403k Authors: Saurabh Menon, Stephen W. Ragsdale Abstract: The cofactor content and mechanism of pyruvate:ferredoxin oxidoreductase (PFOR) are controversial. By using rapid freeze-quench EPR and stopped-flow spectroscopy, the elementary steps that...

Mechanistic Studies on the Mononuclear Zn^II-Containing Metallo-β-lactamase ImiS from Aeromonas sobria

DOI: 10.1021/bi060893t Authors: Narayan Prasad Sharma, Christine E. Hajdin, Sowmya Chandrasekar, Brian Bennett, Ke‐Wu Yang, Michael W. Crowder Abstract: In an effort to understand the reaction mechanism of a B2 metallo-beta-lactamase, steady-state and pre-steady-state...

Reaction of Ferric Cytochrome P450cam with Peracids

DOI: 10.1074/jbc.m501761200 Authors: Tatyana Spolitak, John H. Dawson, David P. Ballou Abstract: Reactions of substrate-free ferric cytochrome P450cam with peracids to generate Fe=O intermediates have previously been investigated with contradictory results. Using...