Low-cost stopped-flow and freeze-quench device for double mixing

DOI: 10.1016/j.ohx.2023.e00409 Authors: Łukasz Bujnowicz, Rafał Pietras, Marcin Sarewicz, Artur Osyczka Abstract: Experiments based on fast reagent mixing and observation of reaction progress are considered a powerful tool for investigating the kinetics of chemical...

(μ-1,2-Peroxo)diiron(III/III) Complex as a Precursor to the Diiron(III/IV) Intermediate X in the Assembly of the Iron-Radical Cofactor of Ribonucleotide Reductase from Mouse

DOI: 10.1021/bi061717n Authors: Danny Yun, Ricardo García‐Serres, Brandon M. Chicalese, Young Hun An, Boi Hanh Huynh, J. Martin Bollinger Abstract: Stopped-flow absorption and freeze-quench electron paramagnetic resonance (EPR) and Mössbauer spectroscopies have been...

Simultaneous reduction of iron–sulfur protein and cytochrome b _L during ubiquinol oxidation in cytochrome bc ₁ complex

DOI: 10.1073/pnas.0607812104 Authors: Jian Zhu, Tsuyoshi Egawa, Syun‐Ru Yeh, Linda Yu, Chang‐An Yu Abstract: The key step of the protonmotive Q-cycle mechanism of the cytochrome bc(1) complex is the bifurcated oxidation of ubiquinol at the Qp site. It was postulated...

Freeze-quenched iron-oxo intermediates in cytochromes P450

DOI: 10.1016/j.bbrc.2005.08.166 Authors: Christiane Jung, Volker Schünemann, Friedhelm Lendzian Abstract: N/A

The effect of pH, ubiquinone depletion and myxothiazol on the reduction kinetics of the prosthetic groups of ubiquinol: Cytochrome c oxidoreductase

DOI: 10.1016/0005-2728(83)90013-0 Authors: Simon de Vries, Simon P. J. Albracht, J.A. Berden, Carla A.M. Marres, E.C. Slater Abstract: N/A