O–H Activation by an Unexpected Ferryl Intermediate during Catalysis by 2-Hydroxyethylphosphonate Dioxygenase

DOI: 10.1021/jacs.6b12147 Authors: Spencer C. Peck, Chen Wang, Laura M. K. Dassama, Bo Zhang, Yisong Guo, Lauren J. Rajakovich, J. Martin Bollinger, Carsten Krebs, Wilfred A. van der Donk Abstract: Activation of O-H bonds by inorganic metal-oxo complexes has been...

Dioxygen Activation at Non-Heme Diiron Centers:  Oxidation of a Proximal Residue in the I100W Variant of Toluene/o-Xylene Monooxygenase Hydroxylase

DOI: 10.1021/bi7017128 Authors: Leslie J. Murray, Ricardo García‐Serres, Michael S. McCormick, Roman Davydov, Sunil Naik, Sun‐Hee Kim, Brian M. Hoffman, Boi Hanh Huynh, Stephen J. Lippard Abstract: At its carboxylate-bridged diiron active site, the hydroxylase...

Redox Function of Tetrahydrobiopterin and Effect of l-Arginine on Oxygen Binding in Endothelial Nitric Oxide Synthase

DOI: 10.1021/bi049026j Authors: Vladimír Berka, Hui-Chun Yeh, De Gao, Kiran Farheen, Ah‐Lim Tsai Abstract: Tetrahydrobiopterin (BH(4)), not dihydrobiopterin or biopterin, is a critical element required for NO formation by nitric oxide synthase (NOS). To elucidate how...

Oxygen Activation by a Mixed-Valent, Diiron(II/III) Cluster in the Glycol Cleavage Reaction Catalyzed by myo-Inositol Oxygenase

DOI: 10.1021/bi0526276 Authors: Gang Xing, Eric W. Barr, Yinghui Diao, Lee M. Hoffart, K. Sandeep Prabhu, Ryan J. Arner, C. Channa Reddy, Carsten Krebs, J. Martin Bollinger Abstract: myo-Inositol oxygenase (MIOX) catalyzes the ring-cleaving, four-electron oxidation of...

Kinetic Characterization of an Organic Radical in the Ascarylose Biosynthetic Pathway

DOI: 10.1021/bi961370w Authors: David A. Johnson, George Gassner, Vahe Bandarian, Frank J. Ruzicka, David P. Ballou, George H. Reed, Hung‐wen Liu Abstract: The lipopolysaccharide of Yersinia pseudotuberculosis V includes a 3,6-dideoxyhexose, ascarylose, as the...