Mechanism of Carbon Monoxide Oxidation by the Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase from Clostridium thermoaceticum:  Kinetic Characterization of the Intermediates

DOI: 10.1021/bi970590m Authors: Javier Seravalli, Manoj Kumar, Wei-Ping Lu, Stephen W. Ragsdale Abstract: Carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) from Clostridium thermoaceticum catalyzes (i) the synthesis of acetyl-CoA from a methylated corrinoid...

Mutual synergy between catalase and peroxidase activities of the bifunctional enzyme KatG is facilitated by electron hole-hopping within the enzyme

DOI: 10.1074/jbc.m117.791202 Authors: Olive J. Njuma, Ian Davis, Elizabeth N. Ndontsa, Jessica R. Krewall, Aimin Liu, Douglas C. Goodwin Abstract: KatG is a bifunctional, heme-dependent enzyme in the front-line defense of numerous bacterial and fungal pathogens...

Primary Steps of the Na+-translocating NADH:Ubiquinone Oxidoreductase Catalytic Cycle Resolved by the Ultrafast Freeze-Quench Approach

DOI: 10.1074/jbc.m808984200 Authors: Alexander V. Bogachev, Nikolai Belevich, Yulia V. Bertsova, Michael I. Verkhovsky Abstract: The Na(+)-translocating NADH:ubiquinone oxidoreductase (Na(+)-NQR) is a component of respiratory chain of various bacteria, and it...

Nitric Oxide Dioxygenation Reaction in DevS and the Initial Response to Nitric Oxide in Mycobacterium tuberculosis

DOI: 10.1021/bi1015315 Authors: Erik T. Yukl, Alexandra Ioanoviciu, Santhosh Sivaramakrishnan, Michiko Nakano, Paul R. Ortiz de Montellano, Pierre Moënne‐Loccoz Abstract: DevS and DosT from Mycobacterium tuberculosis (MTB) are paralogous heme-based sensor kinases that...

Substrate-Mediated Oxygen Activation by Homoprotocatechuate 2,3-Dioxygenase: Intermediates Formed by a Tyrosine 257 Variant

DOI: 10.1021/bi301114x Authors: Michael M. Mbughuni, Katlyn K. Meier, Eckard Münck, John D. Lipscomb Abstract: Homoprotocatechuate (HPCA; 3,4-dihydroxyphenylacetate or 4-carboxymethyl catechol) and O(2) bind in adjacent ligand sites of the active site Fe(II) of...