Detection of a Tryptophan Radical as an Intermediate Species in the Reaction of Horseradish Peroxidase Mutant (Phe-221 → Trp) and Hydrogen Peroxide

DOI: 10.1074/jbc.273.24.14753 Authors: Atsushi Morimoto, Motomasa Tanaka, Satoshi Takahashi, Koichiro Ishimori, Hiroshi Hori, Isao Morishima Abstract: The crucial reaction intermediate in the reaction of peroxidase with hydrogen peroxide (H2O2), compound I, contains a...

On the Mechanism of a Polyunsaturated Fatty Acid Double Bond Isomerase from Propionibacterium acnes

DOI: 10.1074/jbc.m809060200 Authors: Alena Liavonchanka, M.G. Rudolph, Kai Tittmann, Mats Hámberg, Ivo Feußner Abstract: The catalytic mechanism of Propionibacterium acnes polyunsaturated fatty acid isomerase (PAI) is explored by kinetic, spectroscopic, and...

定住者のいない満洲／「逞しき」朝鮮人 : 中野重治「モスクワ指して」の植民地表象をめぐって

DOI: 10.1007/s00249-003-0306-y Authors: 東周 徐 Abstract: Circular dichroism, nuclear magnetic resonance, electron paramagnetic resonance, UV-vis absorption, and resonance Raman (RR) spectroscopic techniques were employed to study protein and heme structural changes of...

How Is Substrate Halogenation Triggered by the Vanadium Haloperoxidase from Curvularia inaequalis?

DOI: 10.1021/acscatal.3c00761 Authors: Emilie F. Gérard, Thirakorn Mokkawes, Linus O. Johannissen, Jim Warwicker, Reynard Spiess, Christopher F. Blanford, Sam Hay, Derren J. Heyes, Sam P. de Visser Abstract: Vanadium haloperoxidases (VHPOs) are unique enzymes in...

Rapid Freeze-Quench EPR Spectroscopy: Improved Collection of Frozen Particles

DOI: 10.1007/s00723-016-0783-7 Authors: Faezeh Nami, Peter Gast, E. J. J. Groenen Abstract: Rapid freeze-quench (RFQ) in combination with electron paramagnetic resonance (EPR) spectroscopy at X-band is a proven technique to trap and characterize paramagnetic...