Gemcitabine 5‘-Triphosphate Is a Stoichiometric Mechanism-Based Inhibitor of Lactobacillus leichmannii Ribonucleoside Triphosphate Reductase:  Evidence for Thiyl Radical-Mediated Nucleotide Radical Formation

DOI: 10.1021/bi972934e Authors: Domingos J. Silva, JoAnne Stubbe, Vicente Sámano, Morris J. Robins Abstract: Ribonucleoside triphosphate reductase (RTPR) from Lactobacillus leichmannii utilizes adenosylcobalamin and catalyzes the conversion of nucleoside triphosphates...

A New Reaction for Improved Calibration of EPR Rapid-Freeze Quench Times: Kinetics of Ethylene Diamine Tetraacetate (EDTA) Transfer from Calcium(II) to Copper(II)

DOI: 10.1007/s00723-021-01448-6 Authors: Abigail L. Schroeter, Hao Yang, Christopher D. James, Brian M. Hoffman, Peter E. Doan Abstract: N/A

Freeze‐Quench Kinetics

DOI: 10.1002/0470862106.ia340 Authors: Simon de Vries Abstract: Abstract Rapid‐mixing, rapid‐freezing techniques are employed for the study of enzyme or chemical catalytic mechanisms. The techniques aim to provide electronic and molecular structures of transient...

Substrate Binding Is the Rate-limiting Step in Thromboxane Synthase Catalysis

DOI: 10.1074/jbc.m009177200 Authors: Lee-Ho Wang, Ah‐Lim Tsai, Pei-Yung Hsu Abstract: Thromboxane synthase (TXAS) is a « non-classical » cytochrome P450. Without any need for an external electron donor, or for a reductase or molecular oxygen, it uses prostaglandin H2...

1.22 Rapid Mixing Techniques for the Study of Enzyme Catalysis

DOI: 10.1016/b978-0-12-374920-8.00127-2 Authors: Sandra Mitić, S. de Vries Abstract: N/A