Oxygen-Induced Radical Intermediates in the nNOS Oxygenase Domain Regulated by l-Arginine, Tetrahydrobiopterin, and Thiol

DOI: 10.1021/bi701677r Authors: Vladimír Berka, Lee-Ho Wang, Ah-Lim Tsai Abstract: Fully coupled nitric oxide synthase (NOS) catalyzes formation of nitric oxide (NO), l-citrulline, NADP+, and water from l-arginine, NADPH, and oxygen. Uncoupled or partially coupled NOS...

Hydrogen Atom Exchange between 5‘-Deoxyadenosine and Hydroxyethylhydrazine during the Single Turnover Inactivation of Ethanolamine Ammonia-Lyase

DOI: 10.1021/bi9906219 Authors: Vahe Bandarian, Russell R. Poyner, George H. Reed Abstract: The early steps in the single turnover inactivation of ethanolamine ammonia-lyase (EAL) from Salmonella typhimurium by hydroxyethylhydrazine (HEH) have been probed by...

Evidence for a Long-Lived, Cu-Coupled and Oxygen-Inert Disulfide Radical Anion in the Assembly of Metallothionein-3 Cu(I)₄-Thiolate Cluster

DOI: 10.1021/jacs.1c03984 Authors: Jenifer S. Calvo, Rhiza Lyne E. Villones, Nicholas J. York, Ewelina Stefaniak, Grace E. Hamilton, Allison L. Stelling, Wojciech Bal, Brad S. Pierce, Gabriele Meloni Abstract: The human copper-binding protein metallothionein-3 (MT-3)...

Shewanella oneidensis Cytochrome c Nitrite Reductase (ccNiR) Does Not Disproportionate Hydroxylamine to Ammonia and Nitrite, Despite a Strongly Favorable Driving Force

DOI: 10.1021/bi401705d Authors: Matthew D. Youngblut, Daniel J. Pauly, Natalia Stein, Daniel T. Walters, John A. Conrad, Graham R. Moran, Brian Bennett, A. Andrew Pacheco Abstract: Cytochrome c nitrite reductase (ccNiR) from Shewanella oneidensis, which catalyzes the...

Revisiting the Val/Ile Mutation in Mammalian and Bacterial Nitric Oxide Synthases: A Spectroscopic and Kinetic Study

DOI: 10.1021/acs.biochem.6b01018 Authors: Marine Weisslocker-Schaetzel, Mehdi Lembrouk, Jérôme Santolini, Pierre Dorlet Abstract: Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a...