A new technique for multiple re-use of planar patch clamp chips

DOI: 10.1016/j.jneumeth.2012.05.002 Authors: Liyo Kao, Natalia Abuladze, Xuesi M. Shao, K. D. McKeegan, Ira Kurtz Abstract: N/A

Polar Residues in the Second Transmembrane Domain of the Rat P2X2 Receptor That Affect Spontaneous Gating, Unitary Conductance, and Rectification

DOI: 10.1523/jneurosci.4403-09.2009 Authors: Lishuang Cao, Helen Broomhead, Mark T. Young, R. Alan North Abstract: Membrane ion channels activated by extracellular ATP (P2X receptors) are widely distributed in the nervous system. Their molecular architecture is...

The different actions of chloride and potassium on postsynaptic inhibition of an isolated neurone

DOI: 10.1242/jeb.64.2.477 Authors: H. Meyer Abstract: Isolated stretch receptor neurones from freshwater crayfish were examined in solutions containing different concentrations of chloride and potassium. In normal solution the inhibitory reversal potential (Eipsp) of...

Thr³³⁹-to-Serine Substitution in Rat P2X₂Receptor Second Transmembrane Domain Causes Constitutive Opening and Indicates a Gating Role for Lys³⁰⁸

DOI: 10.1523/jneurosci.4036-07.2007 Authors: Lishuang Cao, Mark T. Young, Helen Broomhead, Samuel J. Fountain, R. Alan North Abstract: P2X2 receptors are ATP-gated ion channels widely expressed by neurons. Thr339 lies in the second of the two transmembrane domains of...

Xenopus oocyte plasma membrane sheets for FRET analysis

DOI: 10.1152/ajpcell.00435.2006 Authors: Michela Ottolia, Kenneth D. Philipson, Scott John Abstract: Plasma membrane sheets from Xenopus oocytes have been isolated for use in fluorescence resonance energy transfer (FRET) measurements. This system has the following...