{"id":995,"date":"2026-04-15T12:54:17","date_gmt":"2026-04-15T12:54:17","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=995"},"modified":"2026-04-15T12:54:17","modified_gmt":"2026-04-15T12:54:17","slug":"extended-depth-of-field-microscopy-with-a-high-speed-deformable-mirror","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=995","title":{"rendered":"Extended depth-of-field microscopy with a high-speed deformable mirror"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1364\/ol.42.000995\" target=\"_blank\" rel=\"noopener\">10.1364\/ol.42.000995<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas G. Bifano, J\u00e9r\u00f4me Mertz<\/p>\n<h4>Abstract:<\/h4>\n<p>We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1364\/ol.42.000995 Authors: William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas G. Bifano, J\u00e9r\u00f4me Mertz Abstract: We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-995","post","type-post","status-publish","format-standard","hentry","category-m470-leis"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/995","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=995"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/995\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=995"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=995"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=995"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}