{"id":950,"date":"2026-04-15T12:58:17","date_gmt":"2026-04-15T12:58:17","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=950"},"modified":"2026-04-15T12:58:17","modified_gmt":"2026-04-15T12:58:17","slug":"immunochemical-identification-of-exposed-regions-of-the-nak%e2%80%90atpase-%ce%b1%e2%80%90subunit","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=950","title":{"rendered":"Immunochemical identification of exposed regions of the Na<sup>+<\/sup>,K<sup>+<\/sup>\u2010ATPase \u03b1\u2010subunit"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1016\/0014-5793(93)81048-5\" target=\"_blank\" rel=\"noopener\">10.1016\/0014-5793(93)81048-5<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Haruo Homareda, Yoshine Nagano, Hideo Matsui<\/p>\n<h4>Abstract:<\/h4>\n<p>A polyclonal antibody against the Na + ,K + \u2010ATPase holoenzyme was prepared. This antibody recognized native Na + ,K + \u2010ATPase and inhibited its activity. The peptide fragments corresponding to various regions of the Na + ,K + \u2010ATPase \u03b1\u2010subunit, which were synthesized from the cDNA, were immunoprecipitated with the antibody, and the M32\u2010D75, M158\u2010D197 and M470\u2010V552 fragments (the latter included K508, a putative ATP binding site) were identified as the epitopes. The M267\u2010I442 fragment, which included a phosphorylation site at D376, and the C\u2010terminal one\u2010third of the \u03b1\u2010subunit from M615 to the C\u2010terminus, were not detected using this antibody. These results suggest that at least three regions on the Na + ,K + \u2010ATPase \u03b1\u2010subunit, M32\u2010D75, M158\u2010D197 and M470\u2010V552, cover its exposed regions, and that some of them are essential for ATPase activity.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1016\/0014-5793(93)81048-5 Authors: Haruo Homareda, Yoshine Nagano, Hideo Matsui Abstract: A polyclonal antibody against the Na + ,K + \u2010ATPase holoenzyme was prepared. This antibody recognized native Na + ,K + \u2010ATPase and inhibited its activity. The peptide fragments corresponding to various regions of the Na + ,K + \u2010ATPase \u03b1\u2010subunit, which were synthesized from [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-950","post","type-post","status-publish","format-standard","hentry","category-m-470-ac-dc-sds"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/950","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=950"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/950\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=950"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=950"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=950"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}