10.22159\/ijpps.2016v8i9.11410<\/a><\/p>\nAuthors:<\/h4>\n
Paini Sri Widyawati<\/p>\n
Abstract:<\/h4>\n
<p><strong>Objective: <\/strong>This study was conducted to determine the DPPH free radical scavenging activity of pluchea extract (PE) and its fraction (ethyl acetate (EAF), n-butanol (BF) and aqueous (AF)) and comparing with the its activity of control antioxidant, such as green tea (GE) and rosemary extracts (RE), \u03b1-tocopherol succinate (ATS) and BHT.<\/p><p><strong>Methods<\/strong>:<strong> <\/strong>The antioxidant activity was evaluated by the decrease in absorbance as the result of DPPH\u02d9 color change from purple to yellow at 517 nm by using a stopped-flow spectrometer UV-Vis. AE (antiradical efficiency) was a parameter more exactly used to measure the antioxidant power than EC<sub>50 <\/sub>(efficient concentration to reduce 50% of DPPH concentration). This parameter was determined by EC<sub>50<\/sub> and TEC<sub>50<\/sub> (the time needed to reach the steady state).<\/p><p><strong>Results: <\/strong>PE and its fraction had free radical-scavenging activity compared with control antioxidant. Based on EC<sub>50<\/sub>, the orders of ability to scavenging DPPH radicals was determined as the EAF~ GE>PE ~ ATS ~ RE ~ AF>BHT ~ BF. The results were not exactly because BHT and ATS were potentially as a synthetic antioxidant. Therefore AE parameter was used to determine scavenging activity. The classification order of scavenging activity based on AE was BHT>GE ~ ATS>EAF>RE~ BF~ PE~ AF.<\/p><p><strong>Conclusion: <\/strong>The PE and its fractions had DPPH free radical scavenging activity. Based on EC<sub>50<\/sub> and AE parameters, the EAF showed the strongest DPPH free radical scavenging activity compared with PE and the other fractions.<\/p><\/p>\n","protected":false},"excerpt":{"rendered":"
DOI: 10.22159\/ijpps.2016v8i9.11410 Authors: Paini Sri Widyawati Abstract: <p><strong>Objective: <\/strong>This study was conducted to determine the DPPH free radical scavenging activity of pluchea extract (PE) and its fraction (ethyl acetate (EAF), n-butanol (BF) and aqueous (AF)) and comparing with the its activity of control antioxidant, such as green tea (GE) and rosemary extracts (RE), \u03b1-tocopherol succinate […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-914","post","type-post","status-publish","format-standard","hentry","category-mos200"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/914","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=914"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/914\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=914"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=914"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=914"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}