{"id":876,"date":"2026-04-15T13:09:55","date_gmt":"2026-04-15T13:09:55","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=876"},"modified":"2026-04-15T13:09:55","modified_gmt":"2026-04-15T13:09:55","slug":"a-genetically-encoded-far-red-fluorescent-indicator-for-imaging-synaptically-released-zn-2","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=876","title":{"rendered":"A genetically encoded far-red fluorescent indicator for imaging synaptically released Zn <sup>2+<\/sup>"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1126\/sciadv.add2058\" target=\"_blank\" rel=\"noopener\">10.1126\/sciadv.add2058<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Tianchen Wu, Manoj Kumar, Jing Zhang, Shengyu Zhao, Mikhail Drobizhev, Mason McCollum, Charles T. Anderson, Ying Wang, Antje Pokorny, Xiaodong Tian, Yiyu Zhang, Thanos Tzounopoulos, Hui\u2010wang Ai<\/p>\n<h4>Abstract:<\/h4>\n<p>Synaptic zinc ion (Zn<sup>2+<\/sup>) has emerged as a key neuromodulator in the brain. However, the lack of research tools for directly tracking synaptic Zn<sup>2+<\/sup> in the brain of awake animals hinders our rigorous understanding of the physiological and pathological roles of synaptic Zn<sup>2+<\/sup>. In this study, we developed a genetically encoded far-red fluorescent indicator for monitoring synaptic Zn<sup>2+<\/sup> dynamics in the nervous system. Our engineered far-red fluorescent indicator for synaptic Zn<sup>2+<\/sup> (FRISZ) displayed a substantial Zn<sup>2+<\/sup>-specific turn-on response and low-micromolar affinity. We genetically anchored FRISZ to the mammalian extracellular membrane via a transmembrane (TM) \u237a helix and characterized the resultant FRISZ-TM construct at the mammalian cell surface. We used FRISZ-TM to image synaptic Zn<sup>2+<\/sup> in the auditory cortex in acute brain slices and awake mice in response to electric and sound stimuli, respectively. Thus, this study establishes a technology for studying the roles of synaptic Zn<sup>2+<\/sup> in the nervous system.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1126\/sciadv.add2058 Authors: Tianchen Wu, Manoj Kumar, Jing Zhang, Shengyu Zhao, Mikhail Drobizhev, Mason McCollum, Charles T. Anderson, Ying Wang, Antje Pokorny, Xiaodong Tian, Yiyu Zhang, Thanos Tzounopoulos, Hui\u2010wang Ai Abstract: Synaptic zinc ion (Zn2+) has emerged as a key neuromodulator in the brain. However, the lack of research tools for directly tracking synaptic Zn2+ [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-876","post","type-post","status-publish","format-standard","hentry","category-mos200"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/876","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=876"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/876\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=876"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=876"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=876"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}