10.1002\/adfm.202209185<\/a><\/p>\nAuthors:<\/h4>\n
Shuang Tian, Henny C. van der Mei, Yijin Ren, Henk J. Busscher, Linqi Shi<\/p>\n
Abstract:<\/h4>\n
Abstract Amyloid\u2010fibers help to maintain the structure of infectious biofilms. Disassembly of amyloid\u2010fibers contributes to biofilm\u2010dispersal in the blood\u2010circulation, from which bacteria should be cleared by the host immune system. However, often the immune system is insufficiently prepared for increasing bacterial concentrations in blood, causing septic symptoms. Here, poly(ethylene glycol)\/poly(\u03b2\u2010amino ester), pH\u2010responsive micelles are prepared for co\u2010delivery of (\u2010)\u2010epigallocatechin\u20103\u2010 O \u2010gallate (EGCG) as a dispersant. Hydrophilic EGCG can not be loaded in the micellar core, but is cross\u2010linked in the micellar shell through pH\u2010reversible, boronic\u2010ester binding. Disassembly of micelles under acidic biofilm conditions facilitates release of EGCG and dispersal of biofilm\u2010bacteria. When core\u2010loaded with ciprofloxacin, in\u2010shell EGCG prevents ciprofloxacin\u2010leakage at physiological pH. Bacterial killing by core\u2010loaded ciprofloxacin in Staphylococcus aureus biofilms is enhanced when released in combination with EGCG in vitro and in vivo. In mice, micelles with in\u2010shell EGCG self\u2010target after tail\u2010vein injection to an infection site, yielding dispersal of pathogens with mild septic symptoms and bacterial killing by the immune system. Septic symptoms disappear slightly faster when micelles are additionally core\u2010loaded with ciprofloxacin. Thus, micelles with in\u2010shell EGCG yield balanced biofilm dispersal inducing only mild septic symptoms, which is good news in an era of increasing antibiotic\u2010resistance.<\/p>\n","protected":false},"excerpt":{"rendered":"
DOI: 10.1002\/adfm.202209185 Authors: Shuang Tian, Henny C. van der Mei, Yijin Ren, Henk J. Busscher, Linqi Shi Abstract: Abstract Amyloid\u2010fibers help to maintain the structure of infectious biofilms. Disassembly of amyloid\u2010fibers contributes to biofilm\u2010dispersal in the blood\u2010circulation, from which bacteria should be cleared by the host immune system. However, often the immune system is insufficiently […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19],"tags":[],"class_list":["post-818","post","type-post","status-publish","format-standard","hentry","category-mos500"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/818","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=818"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/818\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=818"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=818"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=818"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}