{"id":687,"date":"2026-04-15T13:26:42","date_gmt":"2026-04-15T13:26:42","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=687"},"modified":"2026-04-15T13:26:42","modified_gmt":"2026-04-15T13:26:42","slug":"critical-comparison-of-the-superoxide-dismutase-like-activity-of-carbon-antioxidant-nanozymes-by-direct-superoxide-consumption-kinetic-measurements","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=687","title":{"rendered":"Critical Comparison of the Superoxide Dismutase-like Activity of Carbon Antioxidant Nanozymes by Direct Superoxide Consumption Kinetic Measurements"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1021\/acsnano.9b04229\" target=\"_blank\" rel=\"noopener\">10.1021\/acsnano.9b04229<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Gang Wu, Vladim\u00edr Berka, Paul J. Derry, Kimberly Mendoza, Eugenia Kakadiaris, Trenton Roy, Thomas A. Kent, James M. Tour, Ah\u2010Lim Tsai<\/p>\n<h4>Abstract:<\/h4>\n<p>The superoxide dismutase-like activity of poly(ethylene glycolated) hydrophilic carbon clusters (PEG-HCCs), anthracite and bituminous graphene quantum dots (PEG-aGQDs and PEG-bGQDs, respectively), and two fullerene carbon nanozymes, tris malonyl-C<sub>60<\/sub> fullerene (C3) and polyhydroxylated-C<sub>60<\/sub> fullerene (C<sub>60<\/sub>-OH<sub><i>n<\/i><\/sub>), were compared using direct optical stopped-flow kinetic measurements, together with three native superoxide dismutases (SODs), CuZnSOD, MnSOD, and FeSOD, at both pH 12.7 and 8.5. Computer modeling including both SOD catalytic steps and superoxide self-dismutation enabled the best choice of catalyst concentration with minimal contribution to the observed kinetic change from the substrate self-dismutation. Biexponential fitting to the kinetic data ranks the rate constant (M<sup>-1<\/sup> s<sup>-1<\/sup>) in the order of PEG-HCCs &gt; CuZnSOD \u2248 MnSOD \u2248 PEG-aGQDs \u2248 PEG-bGQDs &gt; FeSOD \u226b C3 &gt; C<sub>60<\/sub>-OH<sub><i>n<\/i><\/sub> at pH 12.7 and MnSOD &gt; CuZnSOD \u2248 PEG-HCCs &gt; FeSOD &gt; PEG-aGQDs \u2248 PEG-bGQDs \u226b C3 \u2248 C<sub>60<\/sub>-OH<sub><i>n<\/i><\/sub> at pH 8.5. Nonlinear regression of the kinetic model above yielded the same ranking as the biexponential fit, but provided better mechanistic insight. The data obtained by freeze-quench EPR direct assay at pH 12.7 also yield the same ranking as stopped-flow data. This is a necessary assessment of a panel of proclaimed carbon nano SOD mimetics using the same two direct methods, revealing a dramatic, 3-4 orders of magnitude difference in SOD activity between PEG-HCCs\/PEG-GQDs from soluble fullerenes.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1021\/acsnano.9b04229 Authors: Gang Wu, Vladim\u00edr Berka, Paul J. Derry, Kimberly Mendoza, Eugenia Kakadiaris, Trenton Roy, Thomas A. Kent, James M. Tour, Ah\u2010Lim Tsai Abstract: The superoxide dismutase-like activity of poly(ethylene glycolated) hydrophilic carbon clusters (PEG-HCCs), anthracite and bituminous graphene quantum dots (PEG-aGQDs and PEG-bGQDs, respectively), and two fullerene carbon nanozymes, tris malonyl-C60 fullerene (C3) [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-687","post","type-post","status-publish","format-standard","hentry","category-freeze-quench"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/687","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=687"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/687\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=687"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=687"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}