{"id":601,"date":"2026-04-15T13:28:23","date_gmt":"2026-04-15T13:28:23","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=601"},"modified":"2026-04-15T13:28:23","modified_gmt":"2026-04-15T13:28:23","slug":"the-algal-petc-pro171-leu-suppresses-electron-transfer-in-the-cytochrome-b-6-f-complex-under-acidic-lumenal-condition","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=601","title":{"rendered":"The algal PETC-Pro171-Leu suppresses electron transfer in the cytochrome <i> b <sub>6<\/sub> f <\/i> complex under acidic lumenal condition"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1101\/2021.12.15.472847\" target=\"_blank\" rel=\"noopener\">10.1101\/2021.12.15.472847<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Shin\u2010Ichiro Ozawa, F\u00e9lix Buchert, Ruby Reuys, Michael Hippler, Yuichiro Takahashi<\/p>\n<h4>Abstract:<\/h4>\n<p>Abstract Linear photosynthetic electron flow (LEF) produces NADPH and generates a proton electrochemical potential gradient across the thylakoid membrane used to synthesize ATP, both of which are required for CO 2 fixation. As cellular demand for ATP and NADPH are variable, cyclic electron flow (CEF) between PSI and cytochrome b 6 f complex ( b 6 f ) produces extra ATP. The b 6 f regulates LEF and CEF via photosynthetic control, which is a pH-dependent b 6 f slowdown of plastoquinol oxidation at the lumenal site. This protection mechanism is triggered at more alkaline lumen pH in the pgr1 mutant of the vascular plant Arabidopsis thaliana , carrying Pro194Leu in the b 6 f Rieske Iron-sulfur protein. In this work, we introduced pgr1 mutation in the green alga Chlamydomonas reinhardtii ( PETC-P171L ). Consistent with pgr1 phenotype, PETC-P171L displayed an impaired NPQ induction along with slower photoautotrophic growth under high light conditions. Our data provides evidence that the \u0394pH component in PETC-P171L is dependent on oxygen availability. Only under low oxygen conditions the \u0394pH component was sufficient to trigger a phenotype in algal PETC-P171L where the mutant b 6 f was more restricted to oxidize the PQ pool and showed a diminished electron flow through the b 6 f complex. One sentence summary Change of PETC to P171L via site directed mutagenesis alters the pH dependency of the photosynthetic control mechanism<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1101\/2021.12.15.472847 Authors: Shin\u2010Ichiro Ozawa, F\u00e9lix Buchert, Ruby Reuys, Michael Hippler, Yuichiro Takahashi Abstract: Abstract Linear photosynthetic electron flow (LEF) produces NADPH and generates a proton electrochemical potential gradient across the thylakoid membrane used to synthesize ATP, both of which are required for CO 2 fixation. As cellular demand for ATP and NADPH are variable, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[],"class_list":["post-601","post","type-post","status-publish","format-standard","hentry","category-jts-150"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/601","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=601"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/601\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=601"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=601"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=601"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}