{"id":551,"date":"2026-04-15T13:35:57","date_gmt":"2026-04-15T13:35:57","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=551"},"modified":"2026-04-15T13:35:57","modified_gmt":"2026-04-15T13:35:57","slug":"a-radical-route-to-making-methane","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=551","title":{"rendered":"A radical route to making methane"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1126\/science.352.6288.949-q\" target=\"_blank\" rel=\"noopener\">10.1126\/science.352.6288.949-q<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Nicholas S. Wigginton<\/p>\n<h4>Abstract:<\/h4>\n<p>Enzyme Mechanisms Microorganisms are the main drivers of Earth&rsquo;s methane cycle. The enzyme ultimately responsible for biological methane production has an ambiguous mechanism because it involves difficult-to-isolate reaction intermediates. Wongnate et al. used stopped-flow and rapid freeze-quench experiments to trap a methyl radical in the active site of methyl-coenzyme M reductase (see the Perspective by Lawton and Rosenzweig). Spectroscopy demonstrated that cofactor F430 contained Ni(II), consistent with computational results. The final step of methanogenesis thus proceeds through Ni(II)-thiolate and methyl radical intermediates rather than an organometallic methyl-Ni(III) mechanism. Science , this issue p. [953][1];, see also p. [892][2] [1]: \/lookup\/doi\/10.1126\/science.aaf0616 [2]: \/lookup\/doi\/10.1126\/science.aaf7700<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1126\/science.352.6288.949-q Authors: Nicholas S. Wigginton Abstract: Enzyme Mechanisms Microorganisms are the main drivers of Earth&rsquo;s methane cycle. The enzyme ultimately responsible for biological methane production has an ambiguous mechanism because it involves difficult-to-isolate reaction intermediates. Wongnate et al. used stopped-flow and rapid freeze-quench experiments to trap a methyl radical in the active site of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-551","post","type-post","status-publish","format-standard","hentry","category-freeze-quench"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/551","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=551"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/551\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=551"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}