10.1002\/cjoc.200890182<\/a><\/p>\nAuthors:<\/h4>\n
Ye-Zhong Zhang, Jie Dai, Cheng Liu, Xiaoping Zhang, Xinliang Ding, Yi Liu<\/p>\n
Abstract:<\/h4>\n
Abstract Under the simulated physiological condition of an animal body, the interaction between N\u2032 \u2010(1\u2010carboxyethylidene)salicylhydrazide (CESH) and bovine serum albumin (BSA) was investigated by fluorescence spectra, UV\u2010Vis absorption spectra and circular dichroism (CD) spectra. The experiment results showed that the fluorescence of BSA was strongly quenched by CESH because of the formation of a CESH\u2010BSA complex, indicating a static quenching mechanism in the binding process. The modified Stern\u2010Volmer quenching constants ( K a ) were 11.23\u00d710 4 , 7.103\u00d710 4 , 4.934\u00d710 4 and 2.495\u00d710 4 L\u00b7mol \u22121 at 292, 298, 304 and 310 K, respectively. The thermodynamic parameters \u0394 G , \u0394 H and \u0394 S at the four temperatures were calculated according to van’t Hoff equation and the results indicated that hydrogen bonds and van der Waals force played major roles in stabilizing the CESH\u2010BSA complex. The distance r =4.26 nm between the donor BSA and acceptor CESH was obtained according to F?rster’s non\u2010radiative energy transfer theory. The synchronous fluorescence spectral results indicated that the hydrophobicity of tyrosine residue increased while the microenvironment around tryptophan residue had no change. The CD and three\u2010dimensional fluorescence spectral results showed that in the presence of CESH, the \u03b1 \u2010helix content of BSA decreased and the microenvironment and conformation of BSA changed.<\/p>\n","protected":false},"excerpt":{"rendered":"
DOI: 10.1002\/cjoc.200890182 Authors: Ye-Zhong Zhang, Jie Dai, Cheng Liu, Xiaoping Zhang, Xinliang Ding, Yi Liu Abstract: Abstract Under the simulated physiological condition of an animal body, the interaction between N\u2032 \u2010(1\u2010carboxyethylidene)salicylhydrazide (CESH) and bovine serum albumin (BSA) was investigated by fluorescence spectra, UV\u2010Vis absorption spectra and circular dichroism (CD) spectra. The experiment results showed that […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-481","post","type-post","status-publish","format-standard","hentry","category-cesh"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/481","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=481"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/481\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=481"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=481"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=481"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}