{"id":1556,"date":"2025-12-23T10:37:08","date_gmt":"2025-12-23T10:37:08","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=1556"},"modified":"2025-12-23T10:37:08","modified_gmt":"2025-12-23T10:37:08","slug":"a-highly-efficient-sorbitol-dehydrogenase-from-gluconobacter-oxydans-g624-and-improvement-of-its-stability-through-immobilization","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=1556","title":{"rendered":"A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1038\/srep33438\" target=\"_blank\" rel=\"noopener\">10.1038\/srep33438<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Tae-Su Kim, Sanjay K. S. Patel, Chandrabose Selvaraj, Woo-Suk Jung, Cheol-Ho Pan, Yun Chan Kang, Jung-Kul Lee<\/p>\n<h4>Abstract:<\/h4>\n<p>Abstract A sorbitol dehydrogenase (GoSLDH) from Gluconobacter oxydans G624 ( G. oxydans G624) was expressed in Escherichia coli BL21(DE3)-CodonPlus RIL. The complete 1455-bp codon-optimized gene was amplified, expressed, and thoroughly characterized for the first time. GoSLDH exhibited K m and k cat values of 38.9 mM and 3820 s \u22121 toward L-sorbitol, respectively. The enzyme exhibited high preference for NADP + (vs. only 2.5% relative activity with NAD + ). GoSLDH sequencing, structure analyses, and biochemical studies, suggested that it belongs to the NADP + -dependent polyol-specific long-chain sorbitol dehydrogenase family. GoSLDH is the first fully characterized SLDH to date, and it is distinguished from other L-sorbose-producing enzymes by its high activity and substrate specificity. Isothermal titration calorimetry showed that the protein binds more strongly to D-sorbitol than other L-sorbose-producing enzymes, and substrate docking analysis confirmed a higher turnover rate. The high oxidation potential of GoSLDH for D-sorbitol was confirmed by cyclovoltametric analysis. Further, stability of GoSLDH significantly improved (up to 13.6-fold) after cross-linking of immobilized enzyme on silica nanoparticles and retained 62.8% residual activity after 10 cycles of reuse. Therefore, immobilized GoSLDH may be useful for L-sorbose production from D-sorbitol.<\/p>\n","protected":false},"excerpt":{"rendered":"<h4>DOI:<\/h4>\n<div class=\"b-doi\">10.1038\/srep33438<\/div>\n<h4>Authors:<\/h4>\n<div class=\"b-author\">Tae-Su Kim, Sanjay K. S. Patel, Chandrabose Selvaraj, Woo-Suk Jung, Cheol-Ho Pan, Yun Chan Kang, Jung-Kul Lee<\/div>\n<h4>Abstract:<\/h4>\n<div class=\"b-intro\">Abstract A sorbitol dehydrogenase (GoSLDH) from Gluconobacter oxydans G624 ( G. oxydans G624) was expressed in Escherichia coli BL21(DE3)-CodonPlus RIL. The complete 1455-bp codon-optimized gene was amplified, expressed, and thoroughly characterized for the first time. GoSLDH exhibited K m and k cat values of 38.9 mM and 3820 s \u22121&hellip;<\/div>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[25],"tags":[],"class_list":["post-1556","post","type-post","status-publish","format-standard","hentry","category-sp-150-potentiostat"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/1556","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1556"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/1556\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1556"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1556"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1556"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}