{"id":1203,"date":"2026-03-25T14:07:48","date_gmt":"2026-03-25T14:07:48","guid":{"rendered":"https:\/\/citations.tools.bio-logic.fr\/?p=1203"},"modified":"2026-03-25T14:07:48","modified_gmt":"2026-03-25T14:07:48","slug":"a-hybrid-electrochemical-flow-reactor-to-couple-h2-oxidation-to-nadh-regeneration-for-biochemical-reactions","status":"publish","type":"post","link":"https:\/\/citations.tools.bio-logic.fr\/?p=1203","title":{"rendered":"A hybrid electrochemical flow reactor to couple H<sub>2<\/sub> oxidation to NADH regeneration for biochemical reactions"},"content":{"rendered":"<h4>DOI:<\/h4>\n<p><a href=\"https:\/\/doi.org\/10.1002\/elsa.202100012\" target=\"_blank\" rel=\"noopener\">10.1002\/elsa.202100012<\/a><\/p>\n<h4>Authors:<\/h4>\n<p>Wassim El Housseini, Fran\u00e7ois Lapicque, Alain Walcarius, Mathieu Etienne<\/p>\n<h4>Abstract:<\/h4>\n<p>Abstract Beta\u2010nicotinamide adenine dinucleotide (NAD + \/NADH) is an important enzymatic co\u2010factor that can be efficiently regenerated using a rhodium\u2010based catalyst as electron transfer mediator ( ie , [Cp*Rh(bpy)Cl] + , where Cp* = pentamethylcyclopentadienyl and bpy = 2,2\u2010bipyridine). Here, the above mediated regeneration of NADH is implemented in a redox flow bioreactor hybridized with a gas diffusion electrode for hydrogen oxidation. The reactor was initially optimized with respect to rhodium complex and NAD + concentrations, humidification of the hydrogen gas, flow rates of both H 2 gas and electrolytic solution, and solution pH. The integration of an enzymatic reaction consuming the generated NADH was then investigated in a flow process, combining in series the electrochemical reactor to a biochemical cell with immobilized l \u2010lactic dehydrogenase for the conversion of pyruvate to lactate. A high activity was achieved with a turnover number up to 370 h \u22121 for NADH regeneration. Coupled electrochemical regeneration to enzymatic reaction led to total turnover number values of 2000 and 6.3 \u00d7 10 6 for NADH electrochemical regeneration and bioconversion, respectively.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DOI: 10.1002\/elsa.202100012 Authors: Wassim El Housseini, Fran\u00e7ois Lapicque, Alain Walcarius, Mathieu Etienne Abstract: Abstract Beta\u2010nicotinamide adenine dinucleotide (NAD + \/NADH) is an important enzymatic co\u2010factor that can be efficiently regenerated using a rhodium\u2010based catalyst as electron transfer mediator ( ie , [Cp*Rh(bpy)Cl] + , where Cp* = pentamethylcyclopentadienyl and bpy = 2,2\u2010bipyridine). Here, the above [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31],"tags":[],"class_list":["post-1203","post","type-post","status-publish","format-standard","hentry","category-vsp-3-potentiostat"],"_links":{"self":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/1203","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1203"}],"version-history":[{"count":0,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=\/wp\/v2\/posts\/1203\/revisions"}],"wp:attachment":[{"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1203"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1203"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/citations.tools.bio-logic.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1203"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}